Description_x000D_ Application_x000D_ AccuTaq™ LA DNA Polymerase is utilized to amplify DNA sequences including genomic targets larger than 20 kb, as a result of a mixture of high quality Taq polymerase with a proofreading polymerase._x000D_ Produce long DNA amplicons for:_x000D_ • Mutation analysis_x000D_ • Cloning genes_x000D_ • DNA sequencing_x000D_ • cDNA library generation_x000D_ Features and Benefits_x000D_ • Increased fidelity, up to 6.5× that of Taq DNA polymerase_x000D_ • Efficiently and accurately produce amplicons up to 22 kb on genomic templates and up to 40 kb on less complex templates such as lambda or bacterial DNA._x000D_ Packaging_x000D_ The enzyme is provided with an optimized 10× reaction buffer for enhanced amplification of complex templates. A separate vial of DMSO is included. Addition of DMSO in the reaction at a final concentration of 1-4% may increase yield and improve reliability of the system with some complex PCR targets._x000D_ Other Notes_x000D_ AccuTaq LA DNA polymerase is an optimized blend of Sigma′s high quality Taq DNA polymerase and a small amount of an additional polymerase that exhibits 3′→5′ exonuclease or proofreading activity. By blending the Taq with the right amount of this proofreading enzyme, misincorporation errors are corrected, producing PCR amplicons that are longer and more accurate._x000D_ Store AccuTaq LA DNA Polymerase and the 10x Buffer at –20 °C. Store DMSO at room temperature. Melting frozen DMSO at ~30 °C will not affect performance._x000D_ Unit Definition_x000D_ One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74°C._x000D_ Legal Information_x000D_ Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA._x000D_ Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA._x000D_ AccuTaq is a trademark of Sigma-Aldrich Co. LLC