ALCOHOL DEHYDROGENASE FROM SACCHAROMYCES

Description_x000D_ General description_x000D_ We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in fuel cell research. For more information see the article in biofiles._x000D_ Application_x000D_ Alcohol dehydrogenase has been used along with lactic dehydrogenase for the enzymatic reduction of acetaldehyde using sodium(R,S)-[2-3H] lactate. Ethanol concentration can be determined colorimentrically by monitoring the enzymatic reduction of NAD using alcohol dehydrogenase after preremoval of the aldehyde group._x000D_ Packaging_x000D_ 7500, 15000, 30000, 75000, 150000, 300000 units in poly bottle_x000D_ Biochem/physiol Actions_x000D_ ADH (alcohol dehydrogenase) is one of the first enzymes to be isolated and purified. NAD+ is its coenzyme. Three isozymes of yeast ADH, that is, yeast alcohol dehydrogenase-1, 2 and 3 (YADH-1, -2, -3) have been identified. YADH-1 is expressed during anaerobic fermentation, YADH-2 is expressed in the cytoplasm and YADH-3 is localized to the mitochondria. A 141kDa tetramer containing 4 equal subunits. The active site of each subunit contains a zinc atom. Each active site also contains 2 reactive sulfhydryl groups and a histidine residue._x000D_ Isoelectric point: 5.4-5.8_x000D_ Optimal pH: 8.6-9.0_x000D_ Substrates: Yeast ADH is most active with ethanol and its activity decreases as the size of the alcohol increases or decreases. Branched chain alcohols and secondary alcohols also have very low activity._x000D_ KM (ethanol) = 2.1 × 10-2 M_x000D_ KM (methanol = 1.3 × 10-1 M_x000D_ KM (isopropanol) = 1.4 × 10-1 M_x000D_ Inhibitors: Compounds that react with free sulfhydryls, including N-alkylmaleimides and iodoacetamide._x000D_ Zinc chelator inhibitors, including 1,10-phenanthroline,_x000D_ 8-hydroxyquinoline, 2,2′-dipyridyl, and thiourea._x000D_ Substrate analogue inhibitors, including β-NAD analogs, purine and pyrimidine derivatives, chloroethanol, and fluoroethanol._x000D_ Extinction Coefficient: E1% = 14.6 (water, 280 nm)_x000D_ Caution_x000D_ Contains bound β-NAD and β-NADH and is not suitable for the recycling microassay of β-NAD and β-NADH. If you require ADH for this purpose, see Catalog No. A3263._x000D_ Unit Definition_x000D_ One unit will convert 1.0 μmole of ethanol to acetaldehyde per min at pH 8.8 at 25 °C._x000D_ Physical form_x000D_ Solids containing ≤ 2% citrate buffer salts_x000D_ Preparation Note_x000D_ Dissolves in water at a concentration of 1 mg/mL to form a clear to slightly hazy, colorless to faintly yellow colored solution.