Description_x000D_ General description_x000D_ _x000D_ ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies._x000D_ Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them._x000D_ Learn more about ZooMAb here._x000D_ Specificity_x000D_ Clone 5H20 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Histone H3 acetylated on lysines 9 and 14._x000D_ Immunogen_x000D_ KLH-conjugated linear peptide corresponding to the first 21 amino acids (without methionine) surrounding acetylated lysines 9 and 14 of human Histone H3._x000D_ Application_x000D_ Anti-Acetyl Histone H3 (Lys9 and 14), clone 5H20, ZooMAb, Cat. No. ZRB06599, is a recombinant Rabbit Monoclonal Antibody that specifically targets Histone H3 acetylated on Lysines 9 and 14 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Peptide Inhibition, & WB._x000D_ Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Acetyl Histone H3 (Lys9 and 14) in HeLa cells treated with Trichostatin A (TSA)._x000D_ Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Acetyl Histone H3 (Lys9 and 14) in human breast cancer tissue sections._x000D_ Peptide Inhibition Analysis: A 1:1,000 dilution from a representative lot was used with HeLa cells treated with Sodium Butyrate for peptide block analysis._x000D_ Target description_x000D_ Histone H3.1t (UniProt: Q16695; also known as H3/t, H3t, H3/g) is encoded by the HIST3H3 (also known as H3FT) gene (Gene ID: 8290) in human. Histones are highly conserved basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. They play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes, which limits DNA accessibility to the cellular machineries that require DNA as a template. Histone H3 features a main globular domain and a long N-terminal tail, which protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family histone acetyl transferases. Histone H3 can also undergo methylation as well as acetylation on lysine 9. Acetylation on lysine 9 (H3K9ac) is reported to impair methylation at arginine 9 (H3R8me2s). A balance between acetylation and methylation is important for the establishment of specific chromatin structures. Acetylation of lysine 14 (H3K14Ac) is commonly seen in genes that are being actively transcribed into RNA. Histone H3 is reported to be hyperacetylated at lysines 9 and 14 following UV irradiation. Studies have shown that nucleosomes with H3K14ac modification have a higher affinity for purified chromatin remodeling complex RSC (Remodels the Structure of Chromatin) and by anchoring RSC, H3K14Ac plays an important role in the unfolding of strongly positioned nucleosomes during repair of UV damage. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Nicolas, E., et al. (2003). Mol. Cell. Biol. 23(5); 1614-1622; Duan, MR., and Smerdon, MJ (2014). J. Biol. Chem. 289(12); 8353-8363)._x000D_ Physical form_x000D_ Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL._x000D_ Reconstitution_x000D_ 300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type._x000D_ Storage and Stability_x000D_ Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Legal Information_x000D_ ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany