Description General description Anti-Aquaporin 5, rabbit polyclonal, recognizes the ~28 kDa AQP 5 protein in epithelial tissue extracts. It is validated for ELISA, Western blotting, immunofluorescence, and immunohistochemistry. Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~28 kDa AQP5 protein. Recognizes the ~28 kDa AQP 5 protein in epithelial tissue extracts. Immunogen a synthetic peptide corresponding to a 17-amino acid sequence in the cytoplasmic region of rat AQP5 Application ELISA (0.5-1.0 µg/ml) Immunoblotting (1-10 µg/ml) Immunofluorescence (2-20 µg/ml) Immunohistochemistry (2-20 µg/ml) Packaging 50 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. Warning Toxicity: Standard Handling (A) Analysis Note Positive Control Epithelial cells Other Notes Does not cross-react with other AQP proteins. The use of 0.5-1.0% milk in all primary/secondary antibody-enzyme conjugate incubations is recommended in order to suppress non-specific bands. Variables associated with assay conditions will dictate the optimal working dilution. Lee, M.D., et al. 1996. J. Biol. Chem. 271, 8599. Raina, S., et al. 1995. J. Biol. Chem. 270, 1908.