ANTI-FIBROBLAST GROWTH FACTOR-BASIC ANTI

Description_x000D_ General description_x000D_ Anti-Fibroblast Growth Factor-Basic antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the FB-8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Fibroblast growth factors (FGFs) are members of a family of polypeptides. The names FGF acidic and basic are used for FGF-1 and FGF-2, respectively. Acidic and basic fibroblast growth factor (aFGF and bFGF, respectively) are members of a small family of heparin binding growth factors (HBGF). bFGF usually has an apparent mass of about 18 kDa._x000D_ Specificity_x000D_ The antibody reacts specifically with both native and recombinant human FGF2. It does not cross-react with human FGF1._x000D_ Immunogen_x000D_ recombinant human FGF2._x000D_ Application_x000D_ Anti-Fibroblast Growth Factor-Basic antibody has been used in in immunolabelling and western blotting. It may also be used in:_x000D_ • in immunoblotting_x000D_ • in enzyme linked immunosorbent assay (ELISA)_x000D_ • in dot blot_x000D_ • in radio immune assay (RIA)_x000D_ • in neutralization bioassays_x000D_ Used in protein pin array assay to identify the interactive sequences in human αB crystalline protein. The antibody can be used for neutralization of the biological activity of FGF2 in cell culture._x000D_ Physical form_x000D_ Solution, 0.2 μm filtered, in phosphate buffered saline, pH 7.4._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ Fibroblast Growth Factor Basic (also known as bFGF) is a pleotropic cytokine that that is a potent mitogenic factor. On binding to its cognate receptor tyrosine kinase, FGFR, bFGF is capable of activating pathways such as MAPK and PI3K and produce cellular effects in varied cell types. Fibroblast Growth Factor-basic is reported to induce proliferation of fibroblasts, differentiation, wound healing and regeneration, limb formation, tissue remodeling and angiogenesis.