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Description_x000D_
General description_x000D_
Nuclear receptor-interacting protein 1 (UniProt: P48552; also known as Nuclear factor RIP140, Receptor-interacting protein 140) is encoded by the NRIP1 gene (Gene ID: 8204) in human. RIP140 is a nuclear protein that is localized to discrete foci and redistributes to larger nuclear domains upon binding to ligand-bound NR3C1. It is expressed in liver in a circadian manner. RIP140 contains 9 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, which have different affinities for nuclear receptors. The C-terminal LTKTNPILYYMLQK motif is required for ligand-dependent interaction with retinoic acid (RA) receptor-alpha (RARA) and retinoid X receptor beta (RXRB) homodimers and heterodimers, for the corepressor activity, and for the formation of an HDAC3 complex with RARA/RXRB. It also contains at least four autonomous repression domains (RD1-4). RD1 functions via a histone deacetylase (HDAC)-independent mechanism, whereas RD2, RD3 and RD4 can function by HDAC-dependent or independent mechanisms, depending on cell type. RIP140 Acetylation regulates its nuclear translocation and co-repressive activity of RIP140 is regulated by its acetylation, which is shown to abolish its interaction with C-terminal-binding protein 1 (CTBP1). Several glucocorticoid responses are shown to be deregulated by RIP140 possibly via an interaction between the glucocorticoid receptor (GR), which prevents binding of true co-activator with GR. (Ref.: Subramaniam N, et al. (1999). J. Biol. Chem. 274(25):18121-7)._x000D_
Specificity_x000D_
Clone 6D7 detects Nuclear receptor-interacting protein 1 (RIP140) in human, mouse, and rat cells. It targets an epitope with in 178 amino acids from the N-terminal half._x000D_
Immunogen_x000D_
GST-tagged recombinant fragment corresponding to 178 amino acids from the N-terminal half of human Nuclear receptor-interacting protein 1 (RIP140)._x000D_
Application_x000D_
Anti-RIP140, clone 6D7, Cat. No. MABS1917, is a mouse monocloanl antibody that detects RIP140 and has been tested for use in Chromatin Immunoprecipitation (ChIP) and Western Blotting._x000D_
Research Category_x000D_
Signaling_x000D_
Western Blotting Analysis: A representative lot detected RIP140 in Western Blotting applications (Kiskinis, E., et. al. (2007). EMBO J. 26(23):4831-40; Hallberg, M., et. al. (2008). Mol Cell Biol. 28(22):6785-95)._x000D_
Chromatin Immunoprecipitation Analysis: A representative lot detected RIP140 in Chromatin Immunoprecitpiation applications (Hallberg, M., et. al. (2008). Mol Cell Biol. 28(22):6785-95)._x000D_
Target description_x000D_
~160 kDa observed; 126.94 kDa calculated. Uncharacterized bands may be observed in some lysate(s)._x000D_
Physical form_x000D_
Format: Purified_x000D_
Protein G purified_x000D_
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide._x000D_
Storage and Stability_x000D_
Stable for 1 year at 2-8°C from date of receipt._x000D_
Disclaimer_x000D_
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_
Quality_x000D_
Evaluated by Western Blotting in MCF7 cell lysate._x000D_
Western Blotting Analysis: 1 µg/mL of this antibody detected RIP140 in 10 µg of MCF7 cell lysate._x000D_
Other Notes_x000D_
Concentration: Please refer to lot specific datasheet.
- Related Categories Alphabetical Index, Antibodies, Primary Antibodies, RH-RN clone