Description Specificity Directed against the Thy 1.1 antigenic determinant which is a monomorphic determinant within rat strains but polymorphic in the mouse (positive to AKR strain, negative to CVA and BALB/c). Thy-1 antigen is present on a variety of cell types including thymocytes, neuronal cells, T-lymphocytes (mouse), stem cells and immature B cells (rat) and connective tissue (Campbell, 1981). Thy-1 is a glycoprotein, 112 amino acids, which is homologous to immunoglobulin domains (Williams & Gagnon, 1982). MAB1406 can be coupled to sepharose 4-B and used to purify mouse Thy-1.1 and rat Thy-1 antigens (Campbell, 1981; Williams & Gagnon, 1982). MAB1406 can also be used to study the killing of Thy-1 tumor cells with antibody-toxin conjugates (Neville, 1982). Immunogen Rat Thy-1 Application Anti-Thy-1.1 Antibody, clone OX-7 detects level of Thy-1.1 & has been published & validated for use in FC, IC, IH. Flow Cytometry: 1:10-1:100. Use 10 μl per 10E6 thymocytes. Immunohistochemistry on frozen sections. Immunocytochemistry Optimal working dilutions must be determined by end user. Research Category Inflammation & Immunology Research Sub Category Immunoglobulins & Immunology Physical form Format: Purified Protein A Purfied Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative. Storage and Stability Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Disclaimer Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Quality Tested Analysis Note Control Thymocytes, T-lymphocytes Other Notes Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.