Description; Application; Bathophenanthrolinedisulfonic acid disodium salt hydrate has been used to measure iron to determine the solubility of iron in the biological sample.[5] It has been used as an iron chelator to grow plants without Fe-EDTA (ethylenediaminetetraacetic acid).[6]; Biochem/physiol Actions; Bathophenanthrolinedisulfonic acid is a metal chelator.[1] It permits iron determination to be made in aqueous medium without need for solvent extraction. It forms a complex with Fe2+ and makes it unavailable to Fe2+ transporter, thereby inhibiting the transport of iron into chloroplasts.[2] Bathophenanthrolinedisulfonic acid is also used in the assay for determining intracellular glutathione.[3][4]