Description; Application; BCIP/NBT solution, premixed has been used: • as a substrate to develop dot-blots[1] • as a substrate to reveal alkaline phosphatase in the immunohistochemical analysis[2] • to detect specific immune complexes in western blot analysis[3][4][5]; Other Notes; 0.48 mM NBT, 0.56 mM BCIP, 10 mM Tris and 59.3 mM MgCl2, pH approx. 9.2; Substrates; Substrate for visualizing alkaline phosphatase conjugates in Western blotting.; Legal Information; BCIP is a registered trademark of Sigma-Aldrich Co. LLC