Description_x000D_ Application_x000D_ β-Glucuronidase Type L-II has been shown to be a superior enzyme for the hydrolysis of drug-glucuronides from urine._x000D_ New Technical Article Comparing Performance of Different Enzymes_x000D_ Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine_x000D_ The enzyme from patella vulgata is reported to be more effective in hydrolyzing opioid-glucuronides than the Helix pomatia, bovine liver and E. coli enzymes_x000D_ Packaging_x000D_ 100000, 250000, 500000, 1000000, 2000000 units in poly bottle_x000D_ Biochem/physiol Actions_x000D_ β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption. Morphine 3-β-D glucuronide was converted to morphine using ß-glucuronidase obtained from Patella vulgate._x000D_ Quality_x000D_ Contains sulfatase activity which is inhibited by 0.1 M phosphate._x000D_ Unit Definition_x000D_ One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).