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Description_x000D_
Application_x000D_
Used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. The use of EDTA buffer, pH 8.5, or other antigen retrieval solutions on FFPE tissue sections improves accessibility of antibodies to tissue antigens. EDTA buffer has been used to heat tissue sections in order to visualize antigens by immunohistochemical analysis.
- Related Categories Antigen Retrieval Solutions, Cell Biology, Core Bioreagents, Hematology and Histology, Life Science Reagents for Immunohistochemistry (IHC), Research Essentials, Tissue ProcessingMore... Quality Level
