Description_x000D_ General description_x000D_ The GenElute HP Endotoxin-Free Plasmid Megaprep Kit offers a simple, rapid, and cost-effective method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. Up to 5 mg of high copy plasmid DNA with = 0.1 Endotoxin Units/μg can be recovered from 200 mL - 1.0 liter LB medium or 200 - 600 mL TB medium of E. coli culture in less than 1.5 hours. Note that the actual yield depends on the strain, the plasmid, and the culture medium used._x000D_ The GenElute HP Endotoxin-Free Plasmid Megaprep Kit offers a simple, rapid, and cost effective method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. Up to 5 mg of high copy plasmid DNA with ≤ 0.1 endotoxin units/μg can be recovered from 200 mL - 1.0 L LB medium or 200 - 600 mL TB medium of E. coli culture in less than 1.5 hours. Note that the actual yield depends on the strain, the plasmid, and the culture medium used._x000D_ High-quality, endotoxin-free DNA is ready for immediate use in downstream applications such as restriction digest, ligation, sequencing, PCR, transformation, and transfection, including endotoxin-sensitive cells._x000D_ Application_x000D_ GenElute™ HP Endotoxin-Free Plasmid Megaprep Kit has been used to purify plasmid DNA._x000D_ High-quality, endotoxin-free DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection, including endotoxin-sensitive cells._x000D_ Features and Benefits_x000D_ • Up to 5 mg of high-copy plasmid DNA with endotoxin levels of ≤0.1 EU/μg_x000D_ • Only 90 minutes from pelleted cells to purified plasmid_x000D_ • Vacuum format with no ethanol precipitation required_x000D_ Other Notes_x000D_ For additional information, please see www.sigma-aldrich.com/genelutehp._x000D_ Legal Information_x000D_ GenElute is a trademark of Sigma-Aldrich Co. LLC