Description_x000D_ General description_x000D_ The Human PSA-total (Prostate Specific Antigen, total) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme- linked immunosorbent assay for the quantitative measurement of human PSA-total in serum, plasma, cell culture supernatants and urine._x000D_ Immunogen_x000D_ Recombinant Human PSA_x000D_ Application_x000D_ For research use only. Not for use in diagnostic procedures._x000D_ Human PSA-total ELISA Kit has been used to determine PSA (prostate specific antigen) level in serum of prostate cancer patients and cancer-free patients._x000D_ Biochem/physiol Actions_x000D_ Prostate-specific antigen (PSA) is an androgen-regulated serine protease specifically produced by prostatic tissue and is secreted into the lumen. PSA in semen cleaves semenogelins present in the seminal coagulum. Total PSA levels are increased in serum of prostate cancer (PCa) patients. PSA functions as a potential biomarker for PCa. Serum protease inhibitors such as α1-antichymotrypsin (α1-ACT), α2-macroglobulin (α2-M), and other acute-phase proteins form irreversible complexes with circulating PSA and hinder proteolytic activity of PSA._x000D_ Other Notes_x000D_ A sample Certificate of Analysis is available for this product._x000D_ Please type the word sample in the text box provided for lot number.