Description_x000D_ General description_x000D_ Nanodisc technology is an approach to render membrane proteins soluble in aqueous solutions in a native-like bilayer environment, where the membrane proteins remain stable and active. The Nanodisc concept is derived from high density lipoprotein (HDL) particles and their primary protein component, apolipoprotein. The Nanodisc is a non-covalent structure of phospholipid bilayer and membrane scaffold protein (MSP), a genetically engineered protein, which mimics the function of Apolipoprotein A-1 (ApoA-1)._x000D_ The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The Membrane Scaffold Protein 1D1 (MSP1D1) variant of MSP1 deletes the first 11 amino acids in the Helix 1 portion (referred to as “H0.5” in the accompanying figure) of the original MSP1 sequence. The MSP1D1 BTN variant of MSP1D1 features an enzymatically biotinylated additional 20-amino acid sequence at the C-terminus of MSP1D1._x000D_ Application_x000D_ For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page._x000D_ Legal Information_x000D_ Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois._x000D_ • 7,691,414 Membrane scaffold proteins_x000D_ • 7,662,410 Membrane scaffold proteins and embedded membrane proteins_x000D_ • 7,622,437 Tissue factor compositions and methods_x000D_ • 7,592,008 Membrane scaffold proteins_x000D_ • 7,575,763 Membrane scaffold proteins and tethered membrane proteins_x000D_ • 7,083,958 Membrane scaffold proteins_x000D_ • 7,048,949 Membrane scaffold proteins