Description_x000D_ General description_x000D_ The gene encoding influenza virus hemagglutinin (HA) precursor is mapped on segment 4. It encodes a 76kDa surface glycoprotein which exists as trimers on the viral envelope._x000D_ Specificity_x000D_ The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells._x000D_ Immunogen_x000D_ synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH_x000D_ Application_x000D_ Monoclonal Anti-HA antibody produced in mouse has been used in 293T cells for:_x000D_ • immunoprecipitation in human embryonic kidney 293 cells_x000D_ • immunoblotting in HeLa cells_x000D_ • immunopurification in human embryonic kidney 293 cells_x000D_ • immunostaining for electron microscopy in mouse embryonic fibroblasts_x000D_ • in immunocytochemistry of hippocampal slices_x000D_ Monoclonal Anti-HA antibody produced in mouse has been used in:_x000D_ • Immunoblotting, to study the expression of fusion protein_x000D_ • Coimmunoprecipitation assay_x000D_ • Immunoblotting of lysate protein_x000D_ Storage and Stability_x000D_ For continuous use, store at 2-8 °C for up to one month._x000D_ For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ Hemagglutinin (HA) is the surface glycoprotein of influenza virus. HA is crucial for virus entry and interaction with N-acetylneuraminic acid (Neu5Ac) in host cell surface. HA coordinates with neuraminidase (NA) and facilitates viral entry into host. HA exists as a trimer and each monomer has stalk transmembrane and globular regions. The globular region contains carbohydrate side chains crucial for infecting host._x000D_ Influenza virus hemagglutinin (HA) acts as a key immunogen in avian influenza virus (AIV) vaccines. Activated HA facilitates virus attachment to oligosaccharide receptors on host cell surface and is involved in penetration through fusion following conformational modifications in the endolysosomal compartment. 226 and 228 residues in hemagglutinin (HA) of the H3 subtype play a key role in host range restriction and receptor specificity. Influenza virus hemagglutinin (HA) is responsible for 1918 influenza pandemic.