MONOCLONAL ANTI-PIWIL1, ANTIBODY PROD&

Description_x000D_ General description_x000D_ Monoclonal Anti-PIWIL1 (mouse IgG1 isotype) is derived from the hybridoma PIWIL1-6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal region of human PIWIL1 conjugated to keyhole limpet hemocyanin (KLH). The PIWI-like protein 1 (PIWIL1) belongs to the Argonaute protein family. This gene is located on human chromosome 12q24.33. PIWIL1 protein has 861 amino acid residues and possess a molecular weight of 98.6/kDa. It is expressed in prostate, ovary, brain, liver, heart, kidney and skeletal muscle._x000D_ Immunogen_x000D_ synthetic peptide corresponding to an internal region of human PIWIL1, conjugated to KLH. The corresponding sequence is identical in mouse PIWIL1._x000D_ Application_x000D_ Monoclonal Anti-PIWIL1 antibody produced in mouse has been used in various immunochemical techniques including immunoblotting (~95 kDa) and immunohistochemistry._x000D_ Physical form_x000D_ Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Legal Information_x000D_ ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC_x000D_ Biochem/physiol Actions_x000D_ Piwi proteins and piRNAs have been implicated in epigenetic control of gene expression, transposon silencing and translation regulation. Piwi proteins participates in germline development and gametogenesis of many metazoan species. PIWI-like protein 1 (PIWIL1) is also involved in stem cell proliferation, embryogenesis, growth and development, along with differentiation and maturation in numerous organisms. It can act as a molecular marker for forecasting the prognosis of colorectal cancer (CRC) patients.