Sepax - инновационные продукты и услуги в области жидкостной хроматографии

Вид

MabPurix P45

MabPurix A45

MabPurix A65

Матрица

PMA

Агароза

Агароза

Размер частиц

45 мкм

45 мкм

65 мкм

Лиганд

Щелочно-толерантный

r-белок А

Щелочно-толерантный

r-белок А

Щелочно-толерантный

r-белок А

DBC

50 мг hIgG/mL смолы

(время удержания 4 мин)

70 мг hIgG/mL смолы

(время удержания 5 мин)

50 мг hIgG/mL смолы

(время удержания 5 мин)

Максимальное

обратное давление

1 МПа

0,3 МПа

0,3 МПа

Стабильность pH

2-12

3-12

3-12

Стабильность 0.5 M NaOH

Встряхивать 25 ч,

DBC > 80%

Встряхивать 25 ч,

DBC > 70%

Встряхивать 25 ч,

DBC > 70%

CIP

Вплоть до 0,5 M NaOH

Вплоть до 0,5 M NaOH

Вплоть до 0,5 M NaOH

Separation

Sepax Column

Typical Running Condition

Antibody Charge

Variants

Antibodix WCX NP5

Proteomix SCX NP5

A: 10 mM Phosphate buffer, pH 7,5

B: A + 0,5 M NaCl

Antibody Aggregates

and Fragments

Zenix SEC-300

Zenix-C SEC 300

150 mM Phosphate

buffer (pH 7,0)

ADC DAR

Proteomix HIC Butyl

A: 2,0 M Ammonium Sulfate, 0,025 M Sodium

Phosphate, pH 7,0

B：0,025 M Sodium Phosphate, pH 7,0

C：100% IPA

ADC Free Drug

Zenix-C SEC 80

50 mM NH₄Ac:ACN=80:20

Bispecific Antibody

Mispair, Homodimer

BioMix SEC 300

PB (8,1 mM Na₃PO₄, 1,5 mM K₃PO₄, 2,7 mM KCl, and 137

mM NaCl, pH 7,4)

Addition of up to 300 mM NaCl to optimize

mRNA

SRT SEC-1000

150 mM Phosphate buffer (pH 7,0)

AAV, VLP

SRT SEC-500, 1000, 2000

150 mM Phosphate buffer (pH 7,0)

Full and Empty Capsid

DNA/RNA

Proteomix WAX NP5

Proteomix SAX NP5

A: 20-200 mM Bis-Tri Propane (pH 8-10) or 20-50 mM TRIS (pH 8)

B: 2,0-20 mM MgCl

C: (A+B) + 1,0 M (CH₃)₄NCl (TMAC) or 1,0 M (C₂H₅)₄NCl (TEAC) or 0,5 M NaCl

D: H₂O

Peptide/Insulin/GLP

Zenix SEC 80, 100, 150

Bio C18

(SEC) 0,1% Arginine:ACN:HAc = 65:20:15 (v/v)

(C18) A: 50% ACN (0,1% TFA); B: 90% ACN (0,1% TFA)

IgM

SRT SEC-500

150 mM Phosphate buffer (pH 7,0)

Antibody Titer

Determination and

Quantification

ProAqa Excel

A: 50 mM Sodium Phosphate, 150 mM

NaCl (pH 7,0)

B: 100 mM Glycine (pH 2,5)

Tween Trap

TweenTrap

150 mM Phosphate buffer (pH 7,0)

Tween Quantification

Monomix H2P-SAX

A: 2,0% formic acid in H₂O;

B: 2,0% formic acid in IPA

Poloxamer

Poly RP-100

SRT-C SEC-150

Monomix H2P-SAX

(PolyRP) A: 0,1% TFA, B: 0,1% TFA ACN

(SEC) 20 mM NH₄Ac, pH 7,0;

(H₂P-SAX) A: 2,0% formic acid in H₂O;

B: 2,0% formic acid in IPA;

Carbohydrate

Carbomix Ca (5 μm, 8%,

7,8 x 300 mm)

H₂O

Sugar and Alcohol

Carbomix-H (10 μm, 5%,

7,8 x 300 mm)

2,5 mM H₂SO₄

Sepax

Tosoh

Waters

Agilent

Thermo

Phenomenex

SEC

SRT SEC-100

SRT-C SEC-100

-

-

Agilent Bio

SEC-5 100Å

BioBasic SEC 120

-

SRT SEC-150

SRT-C SEC-150

TSKgel

G2000SWxL

-

Agilent Bio

SEC-5 150Å

-

BioSep-SECS2000

SRT SEC-300

SRT-C SEC-300

TSKgel

G3000SWxL

-

Agilent Bio

SEC-5 300Å

BioBasic SEC 300

MAbPac SEC-1

BioSep-S ECS3000

SRT SEC-500

SRT-C SEC-500

TSKgel

G4000SWxL

Xbridge Protein

BEH 450 SEC

Agilent Bio

SEC-5 500Å

-

BioSep-SECS4000

SRT SEC-1000

SRT-C SEC-1000

-

-

Agilent Bio

SEC-5 1000Å

BioBasic SEC

1000

-

SRT SEC-2000

SRT-C SEC-2000

-

-

Agilent Bio SEC-5 2000Е

-

-

Zenix SEC-80

-

-

-

-

-

Zenix SEC-100

TSKgel Super

SW G2000

Xbridge Protein

BEH 125 SEC

Agilent Bio

SEC-3 100Å

-

-

Zenix SEC-150

TSKgel Super

SW G2000

Xbridge Protein

BEH 125 SEC

Agilent Bio

SEC-3 150Å

-

-

Zenix SEC-300

TSKgel Super

SW G3000

Xbridge Protein BEH

200 SEC

BioResolve SEC mAb

Column

Agilent Bio SEC-3

300Å

Agilent AdvanceBio

SEC300Å

-

Phenomenex

Yarra SEC 3000

Unix-C

SEC-300

TSKgel UPSW3000

Acquity UPLC Protein BEH 200 SEC

-

-

bioZen 1.8um

SEC-3

IEX

Proteomix SCX

TSKgel SP-5PW

Protein-Pak Hi Res IEX SP

BioResolve SCX mAb

Agilent Bio

IEX SCX

ProPac SCX-10

MAbPac SCX

-

Proteomix WCX

TSKgel CM-5PW

Protein-Pak Hi Res

Agilent Bio

IEX WCX

ProPac WCX-10

ProPac Elite WCX

bioZen WCX

Proteomix SAX

TSKgel SuperQ-5PW

TSKgel QAE-2SW

Protein-Pak Hi

Res IEX Q

Agilent Bio

IEX SAX

ProPac SAX-10

-

Proteomix WAX

TSKgel DEAE-

5PW

-

Agilent Bio

IEX WAX

ProPac WAX-10

-

Antibodix

TSKgel CM-STAT

Protein-Pak Hi Res CM

Agilent Bio

IEX WCX

ProPac WCX-10

bioZen WCX

HIC

Proteomix HIC

Phenyl

TSKgel Phenyl-

5PW

-

-

-

-

Proteomix HIC

Butyl

TSKgel Butyl-NPR

Protein-Pak Hi

Res HIC

AdvanceBio

HIC

ProPac HIC-10

MAbPac HIC-10

-

Proteomix HIC

Propyl

-

-

-

-

-

Proteomix HIC

Ethyl

TSKgel Ether-

5PW

-

-

-

-

AFC

ProAqa Excel

TSKgel ProteinA-

5PW

-

Agilent Bio-

Monolith Protein A

MAbPac Protein A

POROS proteinA

-