A-AMANITIN FROM AMANITA PHALLOIDES

Description Amino Acid Sequence Asn-Hyp-Hyi-Trp-Gly-Ile-Gly-Cys [Bridge: 4-8] Application α-Amanitin has been used: • for inducing transcriptional arrest in NT2 cells prior to immunofluorescence assay • to induce nephrotoxicity in mice renal tissues • to induce and analyse genotoxicity in mice bone marrow cells by cell viability assay, comet assay and chromosomal aberration assay Biochem/physiol Actions The major toxic constituent of the mushroom, Amanita phalloides, inhibits eukaryotic RNA polymerase II and III, but does not inhibit RNA polymerase I or bacterial RNA polymerase. Inhibits mammalian protein synthesis. Caution When stored frozen in the dark, retained samples were found to be greater than 99% by HPLC after two years. Reconstitution Store a 1 mg/ml aqueous stock solution frozen at -20°C. It does not degrade with repeated freeze-thaw cycles. Dilute into the appropriate buffer immediately prior to use. Product is destroyed by concentrated acid or base.