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5,5'-DITHIOBIS(2-NITROBENZOIC ACI&
Кат. №: D8130
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Description_x000D_
General description_x000D_
5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent. It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH. Thiolate anion (RS-) reacts with Ellman′s reagent (DTNB2-), to produce 2-nitro-5-thiobenzoate anion (TNB2-) and one mixed disulfide (R-S-TNB-). TNB2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3._x000D_
Application_x000D_
Ellman′s reagent maybe used for acetylcholinesterase assay, and to specifically label cysteine residues. It is a sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues._x000D_
Packaging_x000D_
1, 5, 10, 25, 100 g in poly bottle_x000D_
500 mg in glass insert
- Related Categories Amino Acid Analysis, Amino Acid Analysis Reagents, Analytical and Industrial Enzymes, Biochemicals and Reagents, Chemical Synthesis, Cholinesterase and Organophosphate Detection Reagents, Detection Reagents, Enzymes, Inhibitors, and Substrates, Greener Alternative Products, Peptide Analysis and Characterization, Peptides, Re−engineered ProductsMore... Quality Level