7-AAD READY MADE SOLUTION

Description_x000D_ General description_x000D_ Free form:_x000D_ Excitation: 503 nm (0.01 M phosphate buffer, pH 7.0 containing 0.1 mM EDTA); 550 nm_x000D_ Emission: 675 nm (0.01 M phosphate buffer, pH 7.0 containing 0.1 mM EDTA); 672 nm_x000D_ DNA Complex:_x000D_ Excitation: 543 nm; 555 nm_x000D_ Emission: 655 nm; 665 nm_x000D_ Application_x000D_ Fluorescent DNA stain. A featured product for Apoptosis research._x000D_ Biochem/physiol Actions_x000D_ 7-AAD is used in flow cytometry analysis of viable cells. 7-ADD is a fluorescent indicator that undergoes a spectral shift upon association with DNA. Su, X., et al. used FITC and PE-conjugated antibodies to stain cell surface markers. After cell-surface marker staining, cells were further stained with 10 μg/ml of 7-AAD in PBS on ice for 30 minutes. After washing with PBS twice, the cells were fixed in 1% paraformaldehyde supplemented with 50 μg/ml actinomycin D. Non-apoptotic cells were 7-AAD negative. 7-AAD like its parent molecule, Actinomycin D, is a DNA-intercalator with growth-inhibitory properties._x000D_ 7-amino-actinomycin D (7-AAD) is a DNA dye. It can be used as a replacement for propidium iodide (PI) for the discrimination of dead cells. This dye is ideal for dead cell discrimination in long-lasting experiments, as it is efficiently exempted by intact cells and has a high DNA binding constant._x000D_ Reconstitution_x000D_ Solution of 1 mg/ml in DMSO:H2O (1:1) is stable for at least 2 years at −20 °C without loss of activity. According to Su et al., the provided solution should be diluted to 10 μg/ml (1:100) working concentration in PBS and is therefore sufficient for 100 stain reactions.