ACYLASE I FROM PORCINE KIDNEY, GRADE I,

Description_x000D_ Application_x000D_ Acylase I from porcine kidney has been used to study the acylase I-catalyzed deacetylation of various S-alkyl-N-acetyl-L-cysteines and their carbon and oxygen analogues . Acylase I may be useful to catalyze N-acetyl amino acids to enantiomerically pure L-amino acids ._x000D_ Packaging_x000D_ 1, 5 g in poly bottle_x000D_ 100, 500 mg in poly bottle_x000D_ Biochem/physiol Actions_x000D_ Acylase I catalyzes the deacetylation of N-acetyl-L-cysteine and S-alkyl-N-acetyl-L-cysteines. n-Butylmalonic acid is an inhibitor of acylase I. S-alkyl-N-acetyl-L-cysteines with short (C0-C3) and unbranched S-alkyl substituents have been found to be good acylase I substrates ._x000D_ Unit Definition_x000D_ One unit will hydrolyze 1.0 μmole of N-acetyl-L-methionine per hr at pH 7.0 at 25 °C._x000D_ Analysis Note_x000D_ Protein determined by biuret.