AGAROSE, BIOREAGENT, FOR MOLECULAR BIOL&

Description_x000D_ General description_x000D_ Agar belongs to the galactan polysaccharides, which make up the intracellular matrix in red seaweeds/marine algae belonging to class Rhodophyta. Agar is naturally slightly sulfated, and is a complex mixture of polysaccharides. Agarose is a neutral polymer component of agar. Heating of agarose gives rise to solution form which on cooling sets into gel form. Gel formation occurs due to double helical formation from molecular chains, which forms a water-immobilizing network._x000D_ Packaging_x000D_ 10, 25, 50, 100, 250, 500 g in poly bottle_x000D_ Application_x000D_ Routine use agarose is ideal for everyday analysis of nucleic acids by gel electrophoresis or blotting (Northern or Southern) and is also suitable for protein applications such as Ouchterlony and radial immunodiffusion (RID). Has low ethidium bromide and SYBR Green background staining._x000D_ Analysis Note_x000D_ The following is a list of properties associated with our agaroses:_x000D_ Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present._x000D_ Gel strength - the force that must be applied to a gel to cause it to fracture._x000D_ Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature._x000D_ Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.