ALCOHOL OXIDASE FROM CANDIDA BOIDINII

Description_x000D_ Application_x000D_ Alcohol oxidase is used to catalyze the oxidation of short-chain, primary, aliphatic alcohols to their respective aldehydes . It may be used to study methanol metabolism is yeasts, such as Candida, Pichia, and Hansenula. It is useful to study protein translocation into peroxisomes. Product A6941 is from Candida boidinii and is provided as a lyophilized powder._x000D_ Packaging_x000D_ 50, 250 units in glass bottle_x000D_ Biochem/physiol Actions_x000D_ Alcohol oxidase has the highest affinity for methanol. The affinity decreases with increasing chain length of the alkyl (R) group. Alcohol oxidase is primarily localized in the peroxisome but is also found in the cytoplasm. Alcohol oxidase is a 600 kDa homooctomeric flavoprotein with eight equal 74 kDa subunits; each containing a flavin adenine dinucleotide (FAD) molecule. The enzyme shows little activity toward secondary, tertiary, or aromatic alcohols; or aliphatic alcohols with a chain length of more than 5 carbons. The pH range for activity of this product is 6.5-8.5, with the optimum pH being 7.5._x000D_ Unit Definition_x000D_ One unit will oxidize 1.0 μmole of methanol to formaldehyde per min at pH 7.5 at 25 °C._x000D_ Physical form_x000D_ Contains potassium phosphate buffer salts, DTE, and stabilizer