ALPHA 2,6-SIALYLTRANSFERASE FROM PHOTOB&

Description_x000D_ General description_x000D_ Human ST6Gal-I (β-galactoside α-2,6-sialyltransferase 1) is a member of the CAZy family GT29._x000D_ Application_x000D_ Highly active α2-6 sialyltransferase has been used to prepare high levels of disialylated fragment crystals._x000D_ α-2,6-Sialyltransferase from Photobacterium damsela has been used in resialylation and restoration of sialic acids (SAs) in HRT-18G cells._x000D_ Biochem/physiol Actions_x000D_ The terminal step of complex N-glycan biosynthesis is catalysed by α-2,6-sialyltransferase (STs). Bacterial α(2,6)-STs possesses broader acceptor substrate specificity when compared to eukaryotic α(2,6)-STs._x000D_ Sialyltransferase transfers Neu5Ac from CMP-Neu5Ac to the galactosyl terminus of acceptor molecules including glycoproteins, glycolipids, and oligosaccharides._x000D_ Unit Definition_x000D_ One unit will catalyze the formation of 1 μmol Neu-5-Ac-α-2,6-LacMU from CMP-Neu-5-Ac and Lac-β−OMU per minute at 37 °C at pH 8.0._x000D_ Physical form_x000D_ Supplied as a lyophilized powder containing Tris-HCl and NaCl._x000D_ Analysis Note_x000D_ Enzymatic activity assays are performed in Tris-HCl buffer (100 mM, pH 8.0) containing CMP-Neu-5-Ac (1 mM) and Lac-β−OMU (1 mM) at 37 °C for 30 min and analyzed using HPLC with a fluorescence detector (excitation at 325 nm and emission at 372 nm).