Description_x000D_ General description_x000D_ Deposits of amyloid protein in senile plaques near nerve processes are found in the brains of aged humand and cases of Alzheimer′s Disease. The principle component of this extracellular amyloid is beta A4, a 4 kDa peptide derived from a larger amyloid precursor protein (APP), which is widely expressed in the brain and body. The creation, transport and function of these proteins is currently an active area of research._x000D_ Specificity_x000D_ Reacts with pre-A4. The antibody recognizes amino acids 66-81 of the N-terminus on the pre-A4 molecule (Hilbich et al., 1993). 22C11 recognizes all three isoforms of APP, immature ~110kDa, sAPP ~120kDa, and mature ~130kDa (Hoffmann et al., 2000). The antibody is known to cross react with APLP2 (Slunt, 1994)._x000D_ Immunogen_x000D_ Epitope: a.a. 66-81 of APP {N-terminus}_x000D_ Purified recombinant Alzheimer precursor A4 (pre A4695) fusion protein._x000D_ Application_x000D_ Anti-APP A4 Antibody, a.a. 66-81 of APP {N-terminus}, clone 22C11 is an antibody against Alzheimer Precursor Protein A4 for use in IF, IH, IH(P) & WB with more than 85 product citations._x000D_ Immunofluorescence_x000D_ Immunohistochemistry: 5-10 μg/mL on 4% paraformaldehyde/15% picric acid perfused and fixed rat spinal cord. 22C11 also works in paraffin embedded tissues but at lower dilutions (1:10-1:20)._x000D_ Western blot: reducing conditions, 10μg/ml._x000D_ Optimal working dilutions and protocols must be determined by the end user._x000D_ Research Category_x000D_ Neuroscience_x000D_ Research Sub Category_x000D_ Neurodegenerative Diseases_x000D_ Target description_x000D_ 110/120/130 kDa_x000D_ Physical form_x000D_ Format: Purified_x000D_ Protein A Purfied_x000D_ Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative._x000D_ Storage and Stability_x000D_ Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Analysis Note_x000D_ Control_x000D_ Brain tissue, brain cell lysates_x000D_ Other Notes_x000D_ Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.