Anti-# Tubulin, cl 10G11 ZooMAb«RbMono

Description_x000D_ General description_x000D_ _x000D_ ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies._x000D_ Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them._x000D_ Learn more about ZooMAb here._x000D_ Specificity_x000D_ Clone 10G11 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects β-tubulin. It targets an epitope within the C-terminal region._x000D_ Immunogen_x000D_ His-tagged recombinant fragment corresponding to 150 amino acids from the C-terminal region of human beta-tubulin._x000D_ Application_x000D_ Anti-Tubulin, clone 10G11 ZooMAb, Cat. No. ZRB1416, is a recombinant Rabbit monoclonal antibody that targets beta-tubulin and has been tested in Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting._x000D_ Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected beta Tubulin in PC12 cell lysate._x000D_ Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected beta- Tubulin in human testis and human kidney tissue sections._x000D_ Affinity Binding Assay: A representative lot of this antibody bound recombinant human beta-tubulin fragment with a KD of 2.3 x 10-6 in an affinity binding assay._x000D_ Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user._x000D_ Target description_x000D_ Tubulin beta chain (UniProt: P07437; also known as Tubulin beta-5 chain) is encoded by the TUBB (also known as TUBB5, OK/SW-cl.56) gene (Gene ID: 203068) in human. Tubulin is the major constituent of microtubules (MTs). MTs are cytoskeletal filaments composed of alpha/beta-tubulin heterodimers that self-assemble head-to-tail to form protofilaments and laterally to form a hollow tube. Alpha/beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Tubulin binds two moles of GTP, one at an exchangeable site on the beta-chain and one at a non-exchangeable site on the alpha-chain. Tubulin dimers constantly polymerize and depolymerize, and MTs can undergo rapid cycles of assembly and disassembly. The first stage of MT formation, the nucleation phase, is slow. In the presence of Mg2+ and GTP, alpha- and beta-tubulins join in an end-to-end manner to form protofilaments with alternating alpha and beta subunits. GTP bound to beta-tubulin is hydrolyzed to GDP during or immediately after polymerization. This weakens the binding affinity of tubulin for adjacent molecules and favors depolymerization that contributes to the dynamic behavior of MTs. MTs also undergo treadmilling, in which tubulin molecules bound to GDP are continually lost from the minus end and are replaced by the addition of GTP-bound tubulin molecules to the plus end of the same MT. Various post-translational modifications, including acetylation, polyglutamylation, polyglycylation, detyrosination, phosphorylation, and palmitoylation, can regulate the polymerization properties of tubulins and/or their interactions with microtubule associated proteins and motor proteins. Beta-Tubulin is reported to undergo phosphorylation on serine 172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals._x000D_ Physical form_x000D_ Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL._x000D_ Reconstitution_x000D_ 0.3 mg/mL after reconstitution at 25μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type._x000D_ Storage and Stability_x000D_ Recommended storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Legal Information_x000D_ ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany