Anti-Apaf-1, clone 13F11

Description_x000D_ General description_x000D_ Apoptotic protease-activating factor 1 (UniProt: O88879; also known as Apaf-1) is encoded by the Apaf-1 gene (Gene ID: 11783) in murine species. Apaf-1 contains a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. It is highly expressed in lung and spleen, weakly in brain and kidney and is not detectable in liver. Under basal conditions it is a monomeric protein, but oligomerizes under apoptotic conditions. The monomeric form is auto-inhibited in a closed conformation through a bound ADP at the nucleotide binding site. Under apoptosis signals ADP is exchanged for ATP and its binding to cytochrome c triggers a conformation change where WD repeat region swings out, which allows the NB-ARC domain to rotate and expose the contact areas for oligomerization. Oligomeric form is a heptameric ring that binds to cytochrome c and ATP and is known as the apoptosome. Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 to mature caspase-9, which then activates effector caspases 3, 6, and 7._x000D_ Specificity_x000D_ Clone 13F11 specifically detects Apaf-1 in mouse and rat. It targets an epitope within the first 97 amino acids in the N-terminal region._x000D_ Immunogen_x000D_ A recombinant fragment corresponding to the first 97 amino acids of mouse Apaf-1._x000D_ Epitope: N-terminus_x000D_ Application_x000D_ Anti-Apaf-1, clone 13F11, is a rat monoclonal antibody that detects Apaf-1 in mouse and rat. It has been tested for use in Western Blotting._x000D_ Research Category_x000D_ Apoptosis & Cancer_x000D_ Western Blotting Analysis: 1 ug/mL from a representative lot detected Apaf-1 in mouse bone marrow tissue lysate._x000D_ Western Blotting Analysis: A representative lot detected Apaf-1 in Western Blotting applications (Vaughn, A.E., et. al. (2007). Cell Death Differ. 14(5):973-81; Wright, K.M., et. al. (2004). J Cell Biol. 167(2):303-13)._x000D_ Target description_x000D_ ~141 kDa observed; 141.00 kDa calculated. Uncharacterized bands may be observed in some lysate(s)._x000D_ Physical form_x000D_ Format: Purified_x000D_ Protein G purified_x000D_ Purified rat monoclonal antibody IgG2a in PBS with 0.02% sodium azide._x000D_ Storage and Stability_x000D_ Stable for 1 year at 2-8°C from date of receipt._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Quality_x000D_ Evaluated by Western Blotting in mouse lymph node tissue lysate._x000D_ Western Blotting Analysis: 1 ug/mL of this antibody detected Apaf-1 in mouse lymph node tissue lysate._x000D_ Linkage_x000D_ Replaces: MAB3504_x000D_ Other Notes_x000D_ Concentration: Please refer to lot specific datasheet.