ANTI-BAX, CLONE 6A7

Description_x000D_ General description_x000D_ Apoptosis regulator Bax (UniProt: Q07812; also known as Bcl-2-like protein 4, Bcl2-L-4) is encoded by the BAX (also known as BCL2L4) gene (Gene ID: 581) in human. Bax is a pro-apoptotic member of the Bcl-2 family of proteins. It displays extensive homology with Bcl-2 and is variably expressed in different cells, usually at very low concentrations in normal cells. Upon induction of apoptosis Bax undergoes a conformational change and translocates from cytosol to mitochondrial membrane. Bax forms homodimers as well as heterodimers with Bcl-2 through common binding motif and the ratio of Bax to Bcl-2 determines the survival or death of cells during apoptosis. BAX is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. Clone 6A7 reacts with multiple isoforms of Bax._x000D_ Specificity_x000D_ Clone 6A7 recognizes BAX in human cells. It targets an epitope with in 13 amino acids from the N-terminal region._x000D_ Immunogen_x000D_ KLH-cojugated linear peptide coprresponding to 13 amino acids from the N-terminal region of human BAX._x000D_ Application_x000D_ Detect using this mouse monoclonal Anti-Bax, clone 6A7 Antibody, Cat. No. MABC1176-25UG, validated for use in Immunoprecipitation, Immunofluorescence and Western Blotting._x000D_ Research Category_x000D_ Apoptosis & Cancer_x000D_ Western Blotting Analysis: A representative lot detected Bax in Western Blotting applications (Xin, M., et. al. (2014). Nat Commun. 5:4935)._x000D_ Immunofluorescence Analysis: A representative lot detected Bax in Immunofluorescence applications (Xin, M., et. al. (2014). Nat Commun. 5:4935)._x000D_ Immunoprecipitation Analysis:A representative lot detected Bax in Immunoprecipitation applications (Xin, M., et. al. (2014). Nat Commun. 5:4935; Hsu, Y.T., et. al. (1998). J Biol Chem. 273(17):10777-83)._x000D_ Physical form_x000D_ Format: Purified_x000D_ Protein G purified_x000D_ Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide._x000D_ Storage and Stability_x000D_ Stable for 1 year at 2-8°C from date of receipt._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Quality_x000D_ Evaluated by Immunoprecipitation in THP-1 cell lysates._x000D_ Immunoprecipitation Analysis: A 1:250 dilution of this antibody detected Bax in THP-1 cell lysates._x000D_ Other Notes_x000D_ Concentration: Please refer to lot specific datasheet.