Description General description Anti-BRCA1 (Ab-2), mouse monoclonal, clone MS13, recognizes ~220 kDa BRCA1 in MCF-7 cells. It is validated for Western blotting, immunofluorescence, immunoprecipitation, and for paraffin sections. Purified mouse monoclonal antibody generated by immunizing mice with the specified iummunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~220 kDa BRCA1 protein. Recognizes the ~220 kDa BRCA1 protein in MCF-7 cells. Does not react with blood group antigens or growth factor receptors such as EGFR. Sold under license of U.S. Patent 5,753,441 and 6,162,897. Immunogen Epitope: within amino acids 1-304 Human recombinant, human BRCA1 Application Immunoblotting (2 µg/ml, chemiluminescence; see application references) Immunofluorescence (see application references) Immunoprecipitation (see application references) Paraffin Sections (see application references) Physical form In 0.05 M sodium phosphate buffer, 0.2% gelatin. Legal Information Sold under license of U.S. Patents 5,753,441 and 6,162,897. Packaging 100 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. Warning Toxicity: Standard Handling (A) Analysis Note Positive Control MCF7 cells Other Notes Antibody should be titrated for optimal results in individual systems. Scully, R., et al. 1996. Science272, 123. Gudas, J.M., et al. 1996. Cell Growth Differ.7, 717. Vaughn, J.P., et al. 1996. Cell Growth Differ.7, 711. Goldgar, D.E. and Reilly, P.R. 1995. Nat. Genet.11, 113. Merajver, S.D., et al. 1995. Clin. Can. Res.1, 539. Merajver, S.D., et al. 1995. Nat. Genet.9, 439. Struewing, J.P., et al. 1995. Nat. Genet.11, 198. Thompson, M.E., et al. 1995. Nat. Genet.9, 444. Futreal, P.A., et al. 1994. Science266, 120. Miki, Y., et al. 1994. Science266, 66.