Anti-C-EBP beta,clone8G18 ZooMAb«Rb Mono

Description_x000D_ General description_x000D_ _x000D_ ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies._x000D_ Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them._x000D_ Learn more about ZooMAb here._x000D_ Specificity_x000D_ Clone 8G18 ZooMAb is a rabbit recombinant monoclonal antibody that specifically detects human CCAAT/enhancer-binding protein beta (C/EBP beta). It targets an epitope with in the N-terminal region._x000D_ Immunogen_x000D_ His-tagged recombinant fragment corresponding to 150 amino acids from the N-terminal half of human CCAAT/enhancer-binding protein beta (C/EBP beta)._x000D_ Application_x000D_ Anti-C-EBP beta, clone 8G18 ZooMAb , Cat. No. ZRB1105, is a highly specific rabbit recombinant monoclonal antibody that targets C-EBP beta and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting._x000D_ Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected C-EBP beta in HeLa, A431, HUVEC, NIH 3T3 and HepG2 cells._x000D_ Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected C-EBP beta in human colon and mouse colon tissue sections._x000D_ Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user._x000D_ Target description_x000D_ CCAAT/enhancer-binding protein beta (UniProt: P17676; also known as C/EBP beta, Liver activator protein, LAP, Liver-enriched inhibitory protein, LIP, Nuclear factor NF-IL6, Transcription factor 5, TCF-5) is encoded by the CEBPB (also known as TCF5) gene (Gene ID: 1051) in human. C/EBP beta is a highly conserved B-Zip family member that acts as a transcription factor regulating the expression of genes involved in immune and inflammatory responses. It is expressed at low levels in the lung, kidney and spleen and is induced by endoplasmic reticulum stress. It can bind to DNA either as a homodimer or a heterodimer with ATF4. C/EBP beta plays a significant role in adipogenesis, as well as in the gluconeogenesis, liver regeneration, and hematopoiesis. During adipogenesis, it is rapidly expressed and, after activation by phosphorylation, induces CEBPA and PPARG, which turn on the series of adipocyte genes giving rise to the adipocyte phenotype. It has a pro-mitotic effect on many cell types, including hepatocytes and adipocytes, but has an anti-proliferative effect on T-cells by repressing MYC expression, facilitating differentiation along the T-helper 2 lineage. C/EBP beta is phosphorylated at threonine 235 by MAPK and CDK2 that serves to prime phosphorylation at threonine 226 and serine 231 by GSK3beta and acquire DNA-binding as well as transactivation activities that are required to induce adipogenesis. However, O-glycosylation at serine 227 and serine 228 is shown to prevent phosphorylation on threonine 235, serine 231, and threonine 226 and reduces its DNA binding activity that delays adipocyte differentiation. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals._x000D_ Physical form_x000D_ Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL._x000D_ Reconstitution_x000D_ 300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type._x000D_ Storage and Stability_x000D_ Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Legal Information_x000D_ ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany