Anti-Calnexin (rabbit polyclonal)

Description_x000D_ General description_x000D_ Calnexin is a Ca++ binding protein found in ER transmembranes that binds to specific n-linked oligosaccharides found on newly synthesized proteins. Calnexin associates with the ER membrane phosphoglycoprotein, signal sequence receptor SSRμ, and has been identified as the ER protein associated with major histocompatibility complex (MHC) class I heavy chain and with the T-cell receptor and membrane immunoglobulin before assembly of these multimeric complexes. Hence, calnexin may represent a molecular chaperone in the ER membrane that is selective for incompletely folded oligomeric membrane proteins newly translocated to the ER._x000D_ Specificity_x000D_ This antibody recognizes Calnexin at the C-terminus._x000D_ Immunogen_x000D_ Epitope: C-terminus_x000D_ KLH-conjugated linear peptide corresponding to the C-terminus of human Calnexin._x000D_ Application_x000D_ Anti-Calnexin Antibody is an antibody against Calnexin for use in WB, IC, IH(P)._x000D_ Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Calnexin in NIH/3T3 cells._x000D_ Immunohistochemistry Analysis: A 1:1,000-2,000 dilution from a representative lot detected Calnexin in human brain and human prostate tissues._x000D_ Research Category_x000D_ Neuroscience_x000D_ Research Sub Category_x000D_ Developmental Neuroscience_x000D_ Target description_x000D_ ~70 kDa observed_x000D_ Physical form_x000D_ Affinity Purfied_x000D_ Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide._x000D_ Storage and Stability_x000D_ Stable for 1 year at 2-8°C from date of receipt._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Quality_x000D_ Evaluated by Western Blot in human brain tissue lysate._x000D_ Western Blot Analysis: A 1:10,000 dilution of this antibody detected Calnexin on 10 µg of human brain tissue lysate._x000D_ Analysis Note_x000D_ Control_x000D_ Human brain tissue lysate_x000D_ Other Notes_x000D_ Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.