ANTI-CD30, DEVELOPED IN GOAT, AFFINITY I

Description; General description; CD30 is a type I transmembrane glycoprotein of the TNF receptor superfamily. The extracellular domain of recombinant mouse CD30/Fc is fused to the carboxyterminal 6X histidine-tagged Fc portion of human IgG1 by a polypeptide linker.; Specificity; Shows no cross-reactivity with recombinant human CD30. The antibody will stimulate the bioactivity of mouse splenic B cell proliferation in the presence of IL-4 and IL-5.; Immunogen; recombinant mouse CD30, extracellular domain, expressed in NSO cells.; Application; Anti-CD30 is suitable for western blotting at a concentration of 0.1-0.2μg/mL and indirect ELISA at a concentration of 0.5-1.0μg/mL; Physical form; Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline; Reconstitution; Reconstitute with 1 mL sterile PBS to yield 0.1 mg/ml solution.; Disclaimer; Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.; Biochem/physiol Actions; Anti-CD30 recognizes recombinant mouse CD30 by immunoblotting and ELISA. In immunoblotting and ELISA, this antibody does not cross-react with recombinant human CD30. Reduced mouse CD30/Fc monomer has a calculated molecular mass of approximately 55 kDa. Due to glycosylation, the recombinant protein migrates as an approximately 90 -100 kDa protein in SDS-PAGE under reducing conditions. CD30 specific IgG is purified from goat serum using mouse CD30 affinity chromatography. CD30 has a critical role in the pathophysiology of Hodgkin′s disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. Anti-CD30 is developed in goat using purified recombinant mouse CD30 extracellular domain, expressed in mouse NSO cells as immunogen.