Description General description Anti-Cdh1 (Ab-2), mouse monoclonal, clone DH01, recognizes the ~55 kDa Cdh-1 protein in Rat-1 cells. It is validated for Western blotting and immunoprecipitation. Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the ~55 kDA Cdh1 protein. Recognizes the ~55 kDa Cdh-1 protein in Rat-1 cells. Immunogen Human recombinant human Cdh1 protein Application Immunoblotting (1 µg/ml) Immunoprecipitation (2 µg/mg protein lysate) Physical form In 10 mM PBS, 0.2% BSA, pH 7.4. Packaging 100 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. Warning Toxicity: Standard Handling (A) Analysis Note Negative Control Normal mouse serum Positive Control Junkat or LS174T cells Other Notes Does not cross-react with other WD repeat containing proteins, including Cdc20. Antibody should be titrated for optimal results in individual systems. Kramer, E.R., et al. 2000 Mol. Biol. Cell11, 1555. Petersen, B.O., et al. 2000 Genes Dev.14, 2330. Pfleger, C.M., et al. 2001 Genes Dev.15, 1759. Sorensen, C.S., et al. 2001 Mol. Cell Biol.21, 3692. Gieffers, C., et al. 1999 Proc. Natl. Acad. Sci. USA96, 11317. Visintin, R., et al. 1997 Science278, 460.