Anti-Erythropoietin,cl 6E5 ZooMAb«Rbmono

Description_x000D_ General description_x000D_ _x000D_ ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies._x000D_ Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them._x000D_ Learn more about ZooMAb here._x000D_ Specificity_x000D_ Clone 6E5 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Erythropoietin._x000D_ Immunogen_x000D_ His-tagged full-length mature recombinant human Erythropoietin (without the signal peptide)._x000D_ Application_x000D_ Anti-Erythropoietin, clone 6E5 ZooMAb, Cat. No. ZRB1366, a recombinant Rabbit Monoclonal Antibody that targets Erythropoietin and is tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting._x000D_ Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Erythropoietin in HUVEC and NIH 3T3 cells._x000D_ Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Erythropoietin in human kidney and rat kidney tissue sections._x000D_ Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user._x000D_ Target description_x000D_ Erythropoietin (UniProt: P01588; also known as Epoetin, EPO) is encoded the EPO gene (Gene ID: 2056) in human. EPO is an acidic glycoprotein that is synthesized with a signal peptide (aa 1-27) that is subsequently cleaved off in the endoplasmic reticulum and the mature protein undergoes glycosylation in the Golgi prior to its release. EPO is mainly produced by peritubular fibroblasts in the renal cortex and to some extent by the liver in adult mammals. In fetal and neonatal mammals, it produced in the liver. EPO is the principle regulator of red blood cell production by stimulating the division and differentiation of bone marrow erythroid progenitor cells in response to anemia and hypoxia. It is induced by ischemic preconditioning by activation of (HIF)-1alpha. It is reported to contribute to angiogenesis in response to ischemia through upregulation of VEGF/VEGFR. It binds to EPO receptor that leads to receptor dimerization and JAK2 activation that results in activation of specific downstream effectors, including STAT1 and STAT3. Endogenous EPO consists of three N-linked polysaccharide groups and one O-linked group. Although its glycosylation slightly reduces its biological activity but is shown to be essential for ensuring its prolonged circulation in the plasma. The N-glycans in its structure protect it from the action of proteases and in the modulation of its receptor binding affinity. Mutations in EPO gene can cause microvascular complications in type II diabetic subjects and erythrocytosis. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Bunn, HF (2013). Cold Spring Harb. Perspect. Med. 3(3); a011619)._x000D_ Physical form_x000D_ Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL._x000D_ Reconstitution_x000D_ 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type._x000D_ Storage and Stability_x000D_ Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Legal Information_x000D_ ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany