Description General description Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERα and ERβ, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ERα and ERβ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERα and ERβ may be regulated by distinct mechanisms even though they share many functional characteristics. Produced in collaboration with Epitomics Specificity Predicted to cross-react with rat and mouse based on sequence homology Recognizes Estrogen Receptor β, long form, does not detect estrogen receptor alpha. Immunogen KLH-conjugated linear peptide corresponding to amino acids 108-127 from the N-terminal half of human estrogen receptor beta. Application Detect Estrogen Receptor β also known as Nuclear receptor subfamily 3 group A member 2 with Anti-Estrogen Receptor β Antibody, clone 68-4 (Rabbit Monoclonal Antibody), that has been demonstrated to work in IP & WB. Research Category Epigenetics & Nuclear Function Research Sub Category Transcription Factors Target description 59 kDa Physical form Format: Purified Protein A Purfied Protein A Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl and 0.05% sodium azide, pH 7.4. Storage and Stability Stable for 1 year at 2-8°C from date of receipt. Disclaimer Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Quality routinely evaluated by immunoblot on RIPA lysates from MCF7 cells Analysis Note Control MCF7 cell extracts Other Notes Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.