Anti-Fibronectin (Ab-3) Mous 1PC x 100UG

Description_x000D_ General description_x000D_ Anti-Fibronectin (Ab-3), mouse monoclonal, clone FBN11, recognizes dimeric fibronectin in SWI56 cells and normal kidney. It is validated for use in WB, IF, IP & IHC (frozen and paraffin sections)._x000D_ Protein G purified mouse monoclonal antibody. Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin._x000D_ Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin in SWI56 cells and normal kidney tissue._x000D_ Immunogen_x000D_ recombinant human fibronectin_x000D_ Application_x000D_ Frozen Sections (0.5-1 µg/ml)_x000D_ Immunoblotting (0.5-1 µg/ml)_x000D_ Immunofluorescence (see comments)_x000D_ Immunoprecipitation (2 µg/mg protein lysate)_x000D_ Paraffin Sections (0.5-1 µg/ml, heat pre-treatment required)_x000D_ Packaging_x000D_ 100 μg in Plastic ampoule_x000D_ Please refer to vial label for lot-specific concentration._x000D_ Warning_x000D_ Toxicity: Standard Handling (A)_x000D_ Analysis Note_x000D_ Positive Control_x000D_ SW 156 cells or normal kidney tissue_x000D_ Other Notes_x000D_ Clark, R.A. 2001. Ann. N.Y. Acad. Sci.936, 355._x000D_ Armstrong, P.B. and Armstrong, M.T. 2000. Biochim. Biophys. Acta1470, O9._x000D_ Barilla, M.L. and Carsons, S.E. 2000. Semin. Arthritis Rheum.29, 252._x000D_ Yamada, K.M. 1989. In Fibronectin, Mosher, D.F., ed., p. 47._x000D_ Rouslahti, E. and Pierschbacher, M.D. 1986. Cell44, 517._x000D_ Pytela, R., et al. 1985. Cell40, 191._x000D_ Exhibits staining of the connective tissue matrix when used in immunohistochemistry. This antibody is suitable for both reduced and non-reduced protein by immunoblotting. For staining of formalin-fixed tissues, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. This antibody has also been reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.