ANTI-FLOTILLIN 1

Description_x000D_ General description_x000D_ Flotillin 1(FLOT1), also known as reggie-2, is an estrogen responsive gene mapped to human chromosome 6p21.33. This gene codes for an integral membrane protein and a component of lipid rafts._x000D_ Flotillins are lipid raft associated proteins expressed mainly in adipose tissue, striated muscle tissues, and lung. It has a crucial role in several cellular processes like phagocytosis, endocytosis, insulin signalling and neuronal regeneration. Anti-flotillin 1 antibody can be used in immunobloting and immunolocalization. Rabbit anti- flotillin 1 antibody reacts specifically with flotillin 1 of rat, mouse and human._x000D_ Immunogen_x000D_ synthetic peptide corresponding to amino acid resides 413-428 of mouse flotillin 1 with an N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in rat and differs by one amino acid in human._x000D_ Application_x000D_ Anti-flotillin 1 antibody can be used in immunofluorescence and western blotting._x000D_ Anti-flotillin 1 antibody produced in rabbit has been used in immunoblot analysis._x000D_ Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below._x000D_ Immunofluorescence (1 paper)_x000D_ Western Blotting (1 paper)_x000D_ Target description_x000D_ Flotillin 1 encodes a caveolae-associated, integral membrane protein. Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction._x000D_ Physical form_x000D_ Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ Flotillin 1 (FLOT1) has a role in endocytosis. It also functions in growth factor signaling and activates mitogen-activated protein kinase signaling. In addition, it is also involved in phagocytosis, regulation of actin cytoskeleton and membrane trafficking. The protein has been associated with tumor progression and is expressed in many cancers, including breast cancer.