Description_x000D_ General description_x000D_ Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It is a glycoprotein and a major class of immunoglobulin. Goat IgG has two subclasses- IgG1 and IgG2._x000D_ Goat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-goat IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Rabbit anti-goat IgG (whole molecule)-peroxidase antibody binds to all goat Igs._x000D_ Specificity_x000D_ Specificity of Anti-Goat IgG- Peroxidase is determined by immunoelectrophoresis (IEP) versus normal goat serum and goat IgG._x000D_ Immunogen_x000D_ Purified goat IgG_x000D_ Application_x000D_ LNCAP cell lysates were analyzed by western blot using HRP conjugated rabbit anti-goat IgG as the secondary at a 1:7500 dilution in TBSt for 1 hour at room temperature._x000D_ Rabbit Anti-Goat IgG (whole molecule)-Peroxidase antibody has been used for immunoblotting assays. The antibody can also be used for direct ELISA (1:40,000), dot blot (1:80,000-1:160,000) and immunohistochemistry (1:200) assays._x000D_ Anti-Goat IgG (whole molecule)–Peroxidase antibody has been used in_x000D_ • western blotting_x000D_ • immunohistochemical and immunofluorescent analysis_x000D_ • visualization of cross-reactivity_x000D_ • protein analysis_x000D_ • indirect ELISA for detection of specific IgG against C. pseudotuberculosis_x000D_ • immunoprecipitation (IP)_x000D_ Physical form_x000D_ Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT_x000D_ Storage and Stability_x000D_ For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ Immunoglobulin G (IgG) glycoprotein antibodies modulates several immune responses. It mainly participates in hypersensitivity type II and type III.