ANTI-HUMAN INSULIN-LIKE GROWTH FACTOR-I,

Description_x000D_ General description_x000D_ Insulin-like growth factors -1 and -2 are important regulators of signalling that mediates growth and metabolism in cells. Insulin-like growth factor -1 shares structural homology with proinsulin and is mainly produced by liver. IGF-1 acts via specific receptor, IGF-1R, a heterodimeric tyrosine kinase receptor that signals to various second messenger pathways such as MAPK, Raf/Ras and PI3K cascades. The effect of IGF-1 is by endocrine, paracrine and autocrine signalling. The effects of IGF-1 signalling are important at many developmental levels from cells to organ to organism, growth, differentiation, anti-apoptosis, proliferation and transformation. Irregular IGF-1 signaling has been implicated in many cancers including melanoma, breast, lung, colon and prostate_x000D_ Anti-IGF-I recognizes human insulin-like growth factor I. The antibody does not react with human IGF-II._x000D_ Specificity_x000D_ The antibody neutralizes the biological activity of recombinant human IGF-I._x000D_ Immunogen_x000D_ recombinant human IGF-I expressed in Escherichia coli_x000D_ Application_x000D_ Anti- IGF-1 antibody may be used for neutralization reactions, the ND50 of the antibody is 3-12 μg/ml. For immunoblotting a working antibody concentration of 0.1-0.2 μg/ml._x000D_ Physical form_x000D_ Lyophilized from a 0.2 μm filtered solution of phosphate buffered saline, pH 7.4, with 5% trehalose._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Preparation Note_x000D_ Affinity isolated by IGF-I affinity chromatography.