Description_x000D_ General description_x000D_ Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2)._x000D_ Specificity_x000D_ Other homologies: Rat (85% sequence homology)._x000D_ This antibody recognizes iNOS/NOS II at the N-terminus._x000D_ Immunogen_x000D_ Epitope: N-terminus_x000D_ GST-tagged recombinant protein corresponding to the N-terminus of mouse iNOS/NOS II._x000D_ Application_x000D_ Detect iNOS/NOS II using this Anti-iNOS/NOS II Antibody, NT validated for use in WB, IP, IH(P)._x000D_ Immunohistochemistry Analysis: 1:50-200 dilution from a representative lot detected iNOS/NOS II in malignant human lung tissues._x000D_ Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated iNOS/NOSII from IFNgamma/LPS treated RAW264.7 cell lysate._x000D_ Research Category_x000D_ Neuroscience_x000D_ Research Sub Category_x000D_ Oxidative Stress_x000D_ Target description_x000D_ ~125 kDa observed_x000D_ Physical form_x000D_ Affinity Purfied_x000D_ Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide._x000D_ Storage and Stability_x000D_ Stable for 1 year at 2-8°C from date of receipt._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Quality_x000D_ Evaluated by Western Blot in IFNgamma/LPS untreated and treated RAW264.7 cell lysates._x000D_ Western Blot Analysis: 0.5 µg/mL of this antibody detected iNOS/NOS II on 10 µg of IFNgamma/LPS untreated and treated RAW264.7 cell lysates._x000D_ Linkage_x000D_ Replaces: 06-573_x000D_ Analysis Note_x000D_ Control_x000D_ IFNgamma/LPS untreated and treated RAW264.7 cell lysates_x000D_ Other Notes_x000D_ Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.