Anti-IRF-1

Description General description Interferon regulatory factor 1 (UniProt: P10914; also known as IRF-1) is encoded by the IRF1 gene (Gene ID: 3659) in human. IRF-1 is a transcriptional regulator that is expressed constitutively in all cells types except early embryonal cells. It can exist as a monomer and as a homodimer. It displays remarkable functional diversity in the regulation of cellular responses, including the regulation of interferon (IFN) and IFN-inducible genes, host response to viral and bacterial infections. It also regulates many genes expressed during hematopoiesis, inflammation, immune responses, cell proliferation and differentiation, and growth arrest and apoptotic cell death following DNA damage. IRF-1 is reported to stimulate p53/TP53-dependent transcription through enhanced recruitment of EP300 leading to increased acetylation of p53/TP53. Its DNA-binging domains are located in the first 115 amino acids of N-terminal region and contains a series of five well-conserved tryptophan-rich repeats. IRF-1 can be phosphorylated by casein kinase 2, which positively regulates its activity. Mutations in IRF1 gene are reported to cause gastric cancer. (Ref.: Dou, L., et al. (2014). Hum. Immunol. 75(11); 1110-1114). Specificity This rabbit polyclonal antibody detects Interferon Regulaotry Factor 1 in human cells. It targets an epitope within 14 amino acids from the N-terminal half. Immunogen KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal half of human Interferon Regulatory Factor 1. Application Anti-IRF-1, Cat. No. ABF188, is a rabbit polyclonal lantibody that detects Interferon regulatory factor 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting. Research Category Inflammation & Immunology Western Blotting Analysis: A 1:500 dilution from a representative lot detected IRF-1 in Jurkat cell lysate. Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected IRF-1 in human stomach cancer and human small intestine tissue sections. Target description ~48 kDa observed; 36.50 kDa calculated. Uncharacterized bands may be observed in some lysate(s). Physical form Affinity Purified Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Storage and Stability Stable for 1 year at 2-8°C from date of receipt. Disclaimer Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Quality Evaluated by Western Blotting in PC12 cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected IRF-1 in PC12 cell lysate. Other Notes Concentration: Please refer to lot specific datasheet.