Anti-MMP-9

Description_x000D_ General description_x000D_ Matrix metalloproteinase-9 (UniProt: P50282; also known as EC: 3.4.24.35, MMP-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB) is encoded by the Mmp9 gene (Gene ID: 81687) in rat. Matrix Metalloproteinases (MMPs) are a family of zinc metallo-endopeptidases secreted by cells and are responsible for much of the turnover of matrix components. MMP-9 is a secreted extracellular matrix metalloproteinase that exhibits a broad range of substrate specificity for native collagens including types IV, V, VII, and X as well as gelatin, proteoglycans, and elastin. It can exist as monomer or disulfide-linked homodimer. MMP-9 is synthesized with a signal peptide (aa 1-19) and a propeptide (aa 20-107) that are subsequently cleaved to produce active enzyme. Its activity can be inhibited by TIMP-1, which binds exclusively to pro-MMP-9 (Kd ~35 nM). MMP-9 contain a fibronectin-like domain inserted into the catalytic domain, presumably to enhance substrate binding. MMP-9 also contains a collagen type-V-like domain, which may enhance substrate binding and specificity. MMP-9 contains a cysteine switch (aa 98-105) where the conserved cysteine binds the catalytic zinc ion and inhibits its enzyme activity. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme._x000D_ Specificity_x000D_ This rabbit polyclonal antibody detects human and rat Matrix metalloproteinase-9. It targets an epitope within 16 amino acids from the N-terminal half._x000D_ Immunogen_x000D_ Epitope: domain of:_x000D_ KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal half of rat Matrix metalloproteinase-9._x000D_ Application_x000D_ Anti-MMP-9, Cat. No. ABT544, is a rabbit polyclonal antibody that detects Matrix Metalloroteinase-9 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.._x000D_ Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected MMP-9 in rat spleen and human bone marrow tissues._x000D_ Research Category_x000D_ Cell Structure_x000D_ Target description_x000D_ ~85 kDa obsereved; 78.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s)._x000D_ Physical form_x000D_ Affinity Purified_x000D_ Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide._x000D_ Storage and Stability_x000D_ Stable for 1 year at 2-8°C from date of receipt._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Quality_x000D_ Evaluated by Western Blotting in lysate from PMA-conditioned HL60 cells._x000D_ Western Blotting Analysis: A 1:500 dilution of this antibody detected MMP-9 in lysate from Phorbol 12-myristate 13-acetate (PMA)-conditioned HL60 cells._x000D_ Other Notes_x000D_ Concentration: Please refer to lot specific datasheet.