Anti-monoandpolyADPRiboseBindingreag

Description General description Anti-mono and poly ADP-ribose binding reagent is a His-tagged recombinant protein fused to rabbit Fc tag, expressed in and purified from Rosetta (DE3)pLysS strain of E. coli (Cat. No. 70956). It is useful for the affinity detection of both poly-and mono-ADP-ribosylated proteins on membranes in a manner similar to antibody-based Western and dot blot analysis. The rabbit Fc tag allows visualization of the binding with conjugated anti-rabbit secondary antibodies. The Fc tag also allows Anti-ADP-ribose binding reagent to be captured on Protein A resins for affinity pull-down applications. Specificity This reagent binds to both mono- and poly-ADP ribosylated proteins. Application Anti-mono- and poly-ADP-ribose binding reagent, Cat. No. MABE1075, is a reagent that targets mono- and poly-ADP-Ribosylated proteins and has been tested in Dot Blots and Western Blotting. Research Category Epigenetics & Nuclear Function Western Blotting Analysis: 0.4 µg/mL of a representative lot detected mono- and poly-ADP ribosylated proteins. Dot Blot Analysis: A representative lot detected mono- and poly-ADP-ribosylated proteins. Dot Blot Analysis: A representative lot detected mono- and poly-ADP-ribosylated proteins (Courtesy of Lee Kraus, University of Texas Southwestern Medical Center). Western Blotting Analysis: A representative lot detected mono-and poly-ribosylated proteins. (Courtesy of Lee Kraus, University of Texas Southwestern Medical Center). Target description Variable depending on the target proteins and the extent of ADP-ribosylation. Physical form Format: Purified Immobilized Metal Affinity Chromatography (IMAC) Purified E. coli recombinant monoclonal binding reagent in buffer containing 10 mM Tris pH 7.5, 0.2 M NaCl, 10 mM Imidazole, 1 mM PMSF, 1 mM -Mercaptoethanol without preservatives with 10% glycerol. Storage and Stability Stable for 1 year at -80°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -80°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Disclaimer Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Quality Evaluated by Gel Electrophoresis. Gel Electrophoresis Analysis: 0.5 µg of this binding reagent was analyzed on GEL Electrophoresis to test for purity. Other Notes Concentration: Please refer to lot specific datasheet.