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Description_x000D_
General description_x000D_
IgG subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm._x000D_
Specificity_x000D_
Goat polyclonal anti-Mouse IgG (Fc specific)–FITC antibody detects all mouse IgG subclasses, it shows no reactivity with mouse IgG Fab fragment or human IgG. Identity and purity of the antibody is established by immunoelectrophoresis, prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion against anti-goat IgG and anti-goat whole serum result in single arcs of precipitation. Goat anti-Mouse IgG is conjugated to FITC and then purified by gel filtration to remove free FITC._x000D_
Immunogen_x000D_
Purified mouse IgG Fc fragment_x000D_
Application_x000D_
Anti-Mouse IgG (Fc specific)-FITC antibody has be used for_x000D_
• Immunofluorescence_x000D_
• Immunohistochemistry_x000D_
• Immunostaining_x000D_
• Confocal microscopy_x000D_
• Flow cytometry_x000D_
Physical form_x000D_
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide._x000D_
Disclaimer_x000D_
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_
Biochem/physiol Actions_x000D_
IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections IgG is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection._x000D_
Anti-Mouse IgG (Fc specific)-FITC antibody is specific for Fc fragment of all mouse IgG subclasses. It shows no reactivity with mouse IgG Fab fragment or human IgG. Goat anti-Mouse IgG is conjugated to FITC and then purified by gel filtration to remove free FITC._x000D_
Other Notes_x000D_
Antibody adsorbed with human IgG._x000D_
Preparation Note_x000D_
Adsorbed to reduce background with human samples.
- Related Categories Alphabetical Index, Antibodies, FITC Antibodies, MO-MR, Mouse Secondary Antibodies and Conjugates, Primary Antibodies, Secondaries listed by Conjugate, Secondaries listed by Target Species, Secondary AntibodiesMore... conjugate