Description_x000D_ General description_x000D_ Muscarinic receptors are members of the G protein coupled receptor family. Five subtypes (M1-M5) of muscarinic receptors have been identified. M1, M2 and M3 activate phospholipases A2, C or D, or tyrosine kinase and M4 and M5 attenuate adenylate cyclase or augment phospholipase A2. Muscarinic receptors are expressed throughout the CNS. M3-receptor is expressed in brain, eye, and heart. ESTs have been isolated from brain, colon, fetus, lung, and prostate libraries. Muscarinic receptors have various presynaptic and postsynaptic effects that are important in both information processing and plastic changes in CNS function. The M3 subtype triggers contraction through an interaction with G(q) proteins, stimulating phosphoinositide hydrolysis and mobilizing Ca2+. M3-receptors appear to be the most functionally important and mediate direct contraction of the detrusor muscle._x000D_ Specificity_x000D_ Rabbit polyclonal anti-Muscarinic Acetylcholine Receptor (M3) antibody recognizes rat, human, mouse Muscarinic Acetylcholine Receptor (M3)._x000D_ Immunogen_x000D_ synthetic peptide corresponding to amino acid residues 461-479 of the third intracellular loop of rat muscarinic acetylcholine receptor M3. This sequence is identical in human, mouse, pig, and bovine._x000D_ Application_x000D_ Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below._x000D_ Immunohistochemistry (1 paper)_x000D_ Rabbit polyclonal anti-Muscarinic Acetylcholine Receptor (M3) antibody is used to tag muscarinic acetylcholine receptor (M3) for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of muscarinic acetylcholine receptor (M3) in information processing and plastic changes in CNS function._x000D_ Physical form_x000D_ Lyophilized from phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% sodium azide._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.