Anti-Myelin Basic Protein Mo

Description General description Purified mouse monoclonal antibody. Recognizes the ~21 kDa myelin basic protein (MBP). Recognizes the ~21 kDa myelin basic protein in rat brain tissue. Does not recognize the 14 kDa form. Recognizes most mammalian species. This Anti-Myelin Basic Protein Mouse mAb (SMI-99) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Frozen Sections, Paraffin Sections for the detection of Myelin Basic Protein. Immunogen Epitope: Within amino acids 131-136 human myelin basic protein Application ELISA (1:1000) Immunoblotting (1:1000) Immunocytochemistry (1:1000) Frozen Sections (1:1000) Paraffin Sections (1:1000) Packaging 100 μL in Plastic ampoule Warning Toxicity: Standard Handling (A) Other Notes Evers, P. and Uylings, H.B. 1997. J. Neurosci. Methods72, 197. Shin, R.W., et al. 1991. Lab. Invest.64, 693. Recognizes MBP from most mammalian species. Does not recognize the ~14 kDa form of MBP. Detects developing and adult myelin and developing oligodendrocytes. Also distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain tissue sections. In combination with Anti-Myelin Basic Protein Mouse mAb (SMI-91) (Cat. No. NE1020) and/or Anti-Myelin Basic Protein Mouse mAb (SMI-94) (Cat. No. NE1018) this antibody is useful for immunocytochemical studies on the progression of normal and pathologic myelination. Antibody should be titrated for optimal results in individual systems.