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Description_x000D_
General description_x000D_
Keratin, type I cytoskeletal 18 (UniProt: P05783; also known as Cell proliferation-inducing gene 46 protein, Cytokeratin-18, CK-18, Keratin-18, K18) is encoded by the KRT18 (also known as CYK18, PIG46) gene (Gene ID: 3875) in human. Cytokeratin-18 is a type I, acidic, protein that is expressed in colon, placenta, and liver. Higher expression levels have been reported in lymph nodes of breast carcinoma. Cytokeratin-18 is involved in the uptake of thrombin-antithrombin complexes by hepatic cells. Upon phosphorylation, it plays a role in filament reorganization. It is proteolytically cleaved by caspases during epithelial cell apoptosis and this cleavage is shown to occur at Asp-238. Phosphorylation of cytokeratin-18 at serine 33 is shown to increase during mitosis in cultured cells and in regenerating liver. Serine 33 phosphorylation is considered to be essential for its association with 14-3-3 proteins and has a role in keratin organization and distribution. Cytokeratin-18 can undergo O-glycosylation, which results in its increased solubility and reduction in its stability. Mutations in KRT18 gene are known to cause liver cirrhosis characterized by severe panlobular liver-cell swelling with Mallory body formation, prominent pericellular fibrosis, and marked deposits of copper._x000D_
Specificity_x000D_
Clone IB4 specifically detects human cytokeratin-18 phosphorylated on serine 33._x000D_
Immunogen_x000D_
KLH-conjugated linear peptide corresponding to 13 amino acids surrounding phosphorylated serine 33 (initiator methionine removed) from the N-terminal region of human cytokeratin-18._x000D_
Application_x000D_
Anti-phospho-cytokeratin-18 (K18) (Ser33), clone IB4, Cat. No. MABT875, is a mouse monoclonal antibody that detects type I cytoskeletal-18 and has been tested for use in Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting._x000D_
Immunocytochemistry Analysis: A representative lot detected phospho-cytokeratin-18 (K18) (Ser33) in immunocytochemistry applications (Ku, N.O., et. al. (1998). EMBO J. 17(7):1892-906)._x000D_
Immunoprecipitation Analysis: A representative lot immunoprecipitated phospho-cytokeratin-18 (K18) (Ser33) in immunoprecipitation applications (Ku, N.O., et. al. (1998). EMBO J. 17(7):1892-906)._x000D_
Immunofluorescence Analysis: A representative lot detected phospho-cytokeratin-18 (K18) (Ser33) in Immunofluorescence applications (Ku, N.O., et. al. (1998). EMBO J. 17(7):1892-906)._x000D_
Western Blotting Analysis: A representative lot detected phospho-cytokeratin-18 (K18) (Ser33) in Western Blotting applications (Yoon, K.H., et. al. (2001). J Cell Biol. 153(3):503-16)._x000D_
Research Category_x000D_
Cell Structure_x000D_
Target description_x000D_
~48 kDa observed; 48.06 kDa calculated. Uncharacterized bands may be observed in some lysate(s)._x000D_
Physical form_x000D_
Format: Purified_x000D_
Protein G purified_x000D_
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide._x000D_
Storage and Stability_x000D_
Stable for 1 year at 2-8°C from date of receipt._x000D_
Disclaimer_x000D_
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_
Quality_x000D_
Evaluated by Western Blotting in lysates from HeLa cells treated with Calyculin A/Okadaic Acid._x000D_
Western Blotting Analysis: 2 µg/mL of this antibody detected phospho-cytokeratin-18 (K18) (Ser33) in lysate from HeLa cells treated with Calyculin A/Okadaic Acid._x000D_
Other Notes_x000D_
Concentration: Please refer to lot specific datasheet.
- Related Categories Alphabetical Index, Antibodies, CT-CZ, Primary Antibodies clone