Anti-p53 (Ab-1) (Pantropic) 1PC X 20UG

Description General description Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant and wild-type p53 proteins. Recognizes the ~53 kDa mammalian wild-type and mutant p53 protein. Does not recognize phosphorylated or acetylated p53. This Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) is validated for use in Frozen Sections, Gel Shift, Immunoblotting, IF, FC, IP for the detection of p53 (Ab-1) (Pantropic). Immunogen Epitope: within amino acids 376-378 of human p53 Mouse partially purified mouse p53 Application Frozen Sections (10 µg/ml, see application references) Gel Shift (see comments) Immunoblotting (10 µg/ml, see application references) Immunofluorescence (1-20 µg/ml, see application references) Flow Cytometry (1-20 µg/ml) Immunoprecipitation (1 μg per sample, see application references) Physical form In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5. Packaging 20, 100 μg in Glass bottle Please refer to vial label for lot-specific concentration. Warning Toxicity: Standard Handling (A) Analysis Note Negative Control SK-OV-3 cells or normal skin Positive Control Breast carcinoma, A431 cells, or p53 standard (Cat. No. 506147) Other Notes El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169. Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855. Legros, Y., et al. 1994. Oncogene9, 2071. Barak, Y., et al. 1993. EMBO J.12, 461. Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491. Kastan, M.B., et al. 1992. Cell71, 587. Lane, D.P. 1992. Nature358, 15. Kastan, M.B., et al. 1991. Cancer Res.51 6304. Crawford, L. and Harlow, E. 1982. J. Virol.41, 709. Harlow, E., et al. 1981. J. Virol.39 861. Originally called clone L21. Does not recognize phosphorylated p53. For a gel shift assay, use Cat. No. OP03L and resuspend in 100 µl buffer. Wild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53, and short incubation times with 35S-Met (≤1 h) will help reduce background. For immunoblotting of wild-type p53, maximize sensitivity by concentrating samples by immunoprecipitation with Cat. No. OP03, then immunoblot using PC35 and chemiluminescent detection. This antibody recognizes the region encompassing Ser376 to Ser378 (see application references) and does not recognize phosphorylated p53 (see application references). Antibody should be titrated for optimal results in individual systems.