Description_x000D_ General description_x000D_ Yes-associated protein 1 (YAP1) is a 65 kDa and a modular adapter protein with multiple protein interaction domains that was originally identified based on its interaction with Src-family tyrosine kinase c-Yes. In addition to a SH3-binding motif, YAP1 contains a proline-rich amino terminus, a WW domain, a coiled-coil and a PDZ-binding motif at the extreme C-terminus. YAP1 binds to p73 through its WW domain and the PPPY motif of p73. This interaction is required for the ability of YAP1 to co-activate p73-responsive genes._x000D_ Specificity_x000D_ Anti-phospho-YAP1 pTyr357 antibody is specific for human phospho-YAP1 pTyr357. The product may react with chicken and mouse phospho-YAP1 pTyr357. In immunoblotting, staining of the phospho-YAP1 (pTyr357) band is specifically inhibited by the immunizing peptide; but no inhibition is observed with the unmodified peptide._x000D_ Immunogen_x000D_ synthetic peptide corresponding to amino acids 351-362 (pTyr357) located in the C-terminal region of human YAP1. The sequence is identical in chicken YAP1 and highly conserved in mouse YAP1 (single amino acid substitution)._x000D_ Application_x000D_ Anti-phospho-YAP1 (pTyr357) antibody produced in rabbit has been used in western blotting and immunofluorescence._x000D_ Physical form_x000D_ Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ Inhibition of Akt potentiates the nuclear re-localization of YAP1 to induce apoptosis by p73._x000D_ YAP1 is a WW domain containing protein that is known to associate with a wide range of transcription factors and functions as a transcriptional co-activator. It may also regulate p73 gene function in response to DNA damage. Alterations in YAP1 have been associated with tumor development_x000D_ Anti-phospho-YAP1 pTyr357 antibody is specific for human phospho-YAP1 pTyr357. The product may react with chicken and mouse phospho-YAP1 pTyr357. In immunoblotting, staining of the phospho-YAP1 (pTyr357) band is specifically inhibited by the immunizing peptide; but no inhibition is observed with the unmodified peptide.