Anti-Pyrin

Description General description Pyrin (UniProt: O15553; also known as Marenostrin) is encoded by the MEFV (also known as MEF, TRIM20) gene (Gene ID: 4210) in human. Pyrin is a homotrimeric protein that is involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. It organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1, ATG16L1, and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Pyrin acts as an autophagy receptor for the degradation of several inflammasome components, including CASP1, NLRP1 and NLRP3, hence it may prevent excessive IL-1B- and IL-18-mediated inflammation. Pyrin is expressed in peripheral blood leukocytes, particularly in mature granulocytes and to a lesser extent in monocytes, but not in lymphocytes. It is also detected in spleen, lung and muscle, probably as a result of leukocyte infiltration in these tissues. Its expression is also detected in several myeloid leukemic, colon cancer, and prostate cancer cell lines. Three isoforms of Pyrin have been described that are produced by alternative splicing. Isoform 1 associates with microtubules and with the filamentous actin of perinuclear filaments and peripheral lamellar ruffles. In pre-apoptotic cells, it co-localizes with PYCARD/ASC in large specks (inflammasome). Isoform 2 is shown to be localized to the nucleus. Specificity This rabbit polyclonal antibody detects Pyrin in human cells. It targets an epitope within 15 amino acids from the N-terminal region. Immunogen KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of human Pyrin, isoform 1. Application Anti-Pyrin, Cat. No. ABF1169, is a rabbit polyclonal antibody that detects human Pyrin and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting. Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Pyrin in human tonsil, human cerebral cortex, human skeletal muscle, and human pancreatic cancer tissues. Target description ~82 kDa observed; 86.44 kDa calculated. Uncharacterized bands may be observed in some lysate(s). Quality Evaluated by Western Blotting in THP-1 cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected Pyrin in 10 µg of THP-1 cell lysate. Other Notes Concentration: Please refer to lot specific datasheet.